Journal: Cell Reports Medicine

Article Title: Therapeutic targeting of interleukin-17C signaling in carcinogenesis of endometriosis

doi: 10.1016/j.xcrm.2025.102464

Figure Lengend Snippet: Effect of IL-17C on malignant transformation in IL-17RE knockdown and overexpressed endometriotic cells (A) qPCR and western blot assay were used to measure the transfection efficiency and IL-17RE expression of si-IL-17RE in 11Z and 12Z. (B) Immunofluorescence staining reveals the effect of rhIL-17C on the expression of PCNA in IL-17RE knockdown 11Z and 12Z. (C–E) (C) Colony formation, (D) adhesion, and (E) migration and invasion were detected in parallel 11Z and 12Z cultures. (F and G) Western blot analysis was used to detect the expression of EMT-related and PI3K pathway key proteins in IL-17RE knockdown cells with or without rhIL-17C for 24 h. (H–J) (H) Immunohistological staining of PCNA, (I) colony formation, and (J) migration and invasion was detected in IL-17RE-overexpressing 11Z and 12Z with and without MOR106 treatment for 24 h. (K) Western blot analysis was used to detect the expression of EMT-related and (L) PI3K pathway key proteins in 11Z and 12Z with and without MOR106 treatment. n = 3 biological replicates. Data are represented as mean ± SEM. ∗ p < 0.05 and ∗∗ p < 0.01 for the indicated comparisons. Differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. ns, not significant. Scale bars, 100 μm.

Article Snippet: Moreover, the effects of rhIL-17C (1234-IL-025, R&D Systems), anti-IL-17RE (NEUT-1237CQ, Creative biolabs, USA), MOR106 (BR2010231, Shanghai bioleaper biotechnology Co. Ltd., China) and LY294002 (HY-10108, MCE) were evaluated on cells respectively.

Techniques: Transformation Assay, Knockdown, Western Blot, Transfection, Expressing, Immunofluorescence, Staining, Migration